Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103
|above: neutrophil & lymphocyte
16 April 1990, rvsd, 14 April 94, 28 Mar 95, 22 March 96, 3 April 97, 2 April 98, 27 Mar 01, 23 Mar 02
|above: eosinophil and lymphocyte
below: platelets and monocyte
Examine the following slide, illustrate the specified view, label
specified features. Note the function of each type of blood cell.
Record the Vernier Scale
of each cell type. The plate numbers are in di Fiore's Atlas
Normal Histology, 9th Ed. Histology of arteries and veins
is at the bottom of this page.
Slide 1. Blood smear, human, 400x, VE: pp. 65, 67 (see also pp. 62-73.)
This slide has been stained with Wright's stain,
complex stain containing eosin (a red stain) and methylene
blue. They allow differential staining of various leukocytes.
Illustrate in a single LARGE view: five or six erythrocytes and platelets, and then at least one each of each class of leukocyte. (Scan at 100x to find the rare leukocytes, then switch to 400x) Record the location of each cell type using the Vernier scale on the mechanical stage. Fill in addresses and copy in the form at bottom of page. Give function of each class (from text or lecture).
erythrocytes most numerous, featureless pink circles
platelets numerous, small, irregular shape and size, reddish (trigger clumping)
|% total leukocytes||vernier
||round nucleus, very little cytoplasm, smallest of leukocytes||immune cells, either B cells or T cells||25 - 33%|
||varied nuclear shape, "like a phone receiver" more cytoplasm, largest of leukocytes||immature macrophage (part of the diffuse reticuloendothelial system of the body)||3 - 7%|
pale cytoplasm, distinct multiple nuclear lobes
also called polymorphonucleocytes = PMNs.
|major class of phagocytes, kill with H2O2, attract other phagocytes||57 - 67%|
||reddish granules in cytoplasm, nucleus bilobed||phagocytose Ag-Ab complexes||1 - 3%|
||cytoplasmic grains dark blue, less numerous, lobes less distinct, larger||Similar to mast cells, contain histamine and heparin||0.5 - 1%|
Other images of leukocytes:
Slide 2. Artery
and vein (H1750) p. 113 & 115, 100x:
Contrasting histology of arteries and veins (See Martini's 5th: p 693):
|1) tunica intima||endothelium (a simple squamous epithelium), attached to internal elastic lamina||endothelium|
|2) tunica media||smooth muscle, a thick band, attached to the external elastic lamina||smooth muscle, a thin band|
|3) tunica externa||adventitia with vasovasorum||adventitia with vasovasorum|
|Arteries are thicker walled, have a smaller diameter, and
open. Veins are larger diameteres, thinner walled, and
You can also see adipose tissue and, in the upper right, two nerves.
|This is a 100x view of an artery. Note again its open lumen and thick wall.|
|At 400x, the artery displays the internal elastic lamina, the thick tunica media, composed of smooth muscle, and the outermost adventitia or tunica externa. Red blood cells can be seen in the lumen.|
|A 400x view of a vein shows little evidence of elasticity of the tunica intima, and a sparce amount of tunica media.|
Label the following in your illustrations of an artery and vein:
internal elastic lamina
VERNIER SCALE ADDRESS OF LEUKOCYTES
Fill in this table, and place in the slide box:
Vernier scale leukocyte addresses form:
Record slide set number, the addresses, your name and the date.
in the slide box.