HISTOLOGY OF

CIRCULATORY SYSTEM

©David B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103

above:  neutrophil & lymphocyte 

 below:  basophil

This page has been accessed Counter times since 27 March 2002. 

16 April 1990, rvsd, 14 April 94, 28 Mar 95, 22 March 96, 3 April 97, 2 April 98, 27 Mar 01, 23 Mar 02
above:  eosinophil and lymphocyte 

below:  platelets and monocyte

Examine the following slide, illustrate the specified view, label the specified features. Note the function of each type of blood cell.  Record the Vernier Scale addresses of each cell type.  The plate numbers are in di Fiore's Atlas of Normal Histology, 9th Ed.  Histology of arteries and veins is at the bottom of this page.

Slide 1. Blood smear, human, 400x, VE: pp. 65, 67 (see also pp. 62-73.)

This slide has been stained with Wright's stain, a complex stain containing eosin (a red stain) and methylene blue.  They allow differential staining of various leukocytes.
Illustrate in a single LARGE view: five or six erythrocytes and platelets, and then at least one each of each class of leukocyte. (Scan at 100x to find the rare leukocytes, then switch to 400x) Record the location of each cell type using the Vernier scale on the mechanical stage. Fill in addresses and copy in the form at bottom of page. Give function of each class (from text or lecture).

erythrocytes most numerous, featureless pink circles

platelets numerous, small, irregular shape and size, reddish (trigger clumping)

LEUKOCYTES:
 

Class

Appearance

Function
% total leukocytes vernier
fore-aft
vernier
lateral
AGRANULOCYTES
lymphocyte
lymphocyte, 1000x

round nucleus, very little cytoplasm, smallest of leukocytes immune cells, either B cells or T cells 25 - 33%    
monocyte
monocyte, 1000x

varied nuclear shape, "like a phone receiver" more cytoplasm, largest of leukocytes immature macrophage (part of the diffuse reticuloendothelial system of the body) 3 - 7%    
GRANULOCYTES
neutrophil
neutrophil, 1000x

numerous, pale cytoplasm, distinct multiple nuclear lobes 
also called polymorphonucleocytes = PMNs.
major class of phagocytes, kill with H2O2, attract other phagocytes 57 - 67%    
eosinophil
eosinophil and lymphocyte, 1000x

reddish granules in cytoplasm, nucleus bilobed phagocytose Ag-Ab complexes 1 - 3%    
basophil
basophil, 1000x

cytoplasmic grains dark blue, less numerous, lobes less distinct, larger Similar to mast cells, contain histamine and heparin 0.5 - 1%

Other images of leukocytes:
basophil:

eosinophils:

monocyte:

neutrophils:

 
 

Slide 2.  Artery and vein (H1750) p. 113 & 115, 100x: endothelium
Contrasting histology of arteries and veins (See Martini's 5th: p 693):
 
TISSUE LAYERS ARTERY VEIN
1) tunica intima endothelium (a simple squamous epithelium), attached to internal elastic lamina endothelium
2) tunica media smooth muscle, a thick band,  attached to the external elastic lamina smooth muscle, a thin band
3) tunica externa adventitia with vasovasorum adventitia with vasovasorum

 Here is a labeled view of an artery and a vein which contrasts their composition.

Arteries are thicker walled, have a smaller diameter, and hold themselves open.  Veins are larger diameteres, thinner walled,  and "floppier."

You can also see adipose tissue and, in the upper right, two nerves.

This is a 100x view of an artery.  Note again its open lumen and thick wall.
At 400x, the artery displays the internal elastic lamina, the thick tunica media, composed of smooth muscle, and the outermost adventitia or tunica externa.  Red blood cells can be seen in the lumen.
A 400x view of a vein shows little evidence of elasticity of the tunica intima, and a sparce amount of tunica media.

 

Label the following in your illustrations of an artery and vein:
    internal elastic lamina
    tunica media
    tunica adventitia
    adipose tissue


VERNIER SCALE ADDRESS OF LEUKOCYTES

Fill in this table, and place in the slide box:

Vernier scale leukocyte addresses form:

Record slide set number, the addresses, your name and the date. Place in the slide box.
 

Slide Set:______ Fore/Aft  Lateral 
neutrophil
   
basophil
   
eosinophil
   
lymphocyte
   
monocyte
   
Student: ______________________Date:________