HISTOLOGY OF THE EYE

©David B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103
 Section through the eye
optic nerve on the left,25x

This page has been accessed Counter times since 24 December 2002. 
11 February 1992, rvsd 26 Dec 93, 31 Dec 94, 31 Dec. 96, 7 Jan 98, 10 Feb 00, 3 Jan 01
  Section through the retina
400x
Follow protocol Notebook Illustrations

This is another slide in which, because of the size of the specimen, you will have to move around to see the entire structure, even at the lowest power. First look at the slide with the naked eye to orient yourself to its gross features: you should be able to see the cornea, lens, cavities, and ciliary bodies.

With the 4x objective, find the sclera and follow it around the entire perimeter, identifying the regions as you come to them and noting the layers in each region. Again, identify all features listed before you begin an illustration. You will make three illustrations, gross anatomy of the anterior portion, a low power view of the rear wall and a high power view of the retina:

Slide 8:  Eye, Monkey, general structure, sagittal section, general features H 1064
(MF 4th, page 343, 345)
 

At 25x: Gross anatomy of section through the eye:
FIBROUS TUNIC:
cornea (anterior 1/6th)
sclera (posterior 5/6ths)
ocular conjunctiva
corneal limbus (cornea & sclera join)
canal of Schlemm (poss. not visible)
VASCULAR TUNIC:
ciliary body:
ciliary muscle
ciliary processes
iris
pupil
NERVOUS TUNIC:
retina
lens (often fragmented)
suspensory ligaments (not visible?)
anterior cavity
anterior chamber
posterior chamber
aqueous humor
posterior cavity
vitreous humor
At 40x: Gross anatomy of anterior portion of the eye:
VASCULAR TUNIC, 60x:
ciliary body:
ciliary muscle
ciliary processes
iris
pupil
VASCULAR TUNIC, 100x:
ciliary body:
ciliary muscle
ciliary processes
iris
pupil
400x view of cornea, left to right:
posterior epithelium (low cuboidal cells)
Corneal stroma withkeratocytes embedded in collagen fibers
anterior epithelium (stratified squamous epithelium)
 
At 100x: cross section through rear wall of eye with optic nerve:
left to right:
optic nerve with parallel axons from ganglion cells
optic disc 
   (note photoreceptors are absent in retina where the optic nerve is attached)
adventitia (orbital fatty tissue)
sclera
choroid, with melanocytes
retina:
    cell bodies of
    photoreceptors
    bipolar cells
    ganglion cells
300x:  view through the rear wall of the eye showing, top to bottom:
Nervous tunic
   ganglion celle bodies
   bipolar cell bodies
   photorecptor cell bodies
Vascular tunic:
   pigmented cells of choroid
   choroid
Fibrous tunic:
   sclera
 
400x view of cross section of retina:
Deep to superficial tissues (left to right):
pigment cells
rods (finer and longer)
cones (thicker and shorter)
outer limiting membrane
nuclei of cones (closer to choroid)
nuclei of rods (further from choroid)
nuclei of:
    horizontal cells (closest to receptors)
    bipolar cells
    amacrine cells (closest to ganglion)
    ganglion cell bodiesMullers fibers (vertical fr ganglion cells)

 
 
 
 
 
 

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