EPITHELIAL TISSUES LAB

©David B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103

This page has been accessed Counter times since 2 October 2001. 
8 October 1991, rvsd 18 October 1994, 12 Sept '95, 3 Oct '96, 16 Dec '96, 17 Sept '97, 11 Sept 99, 28 Sept 01

First review and follow carefully the rules and regulations for microscope use. Also, be familiar with guidelines in Notebook Illustrations for these drawings.

Examine each of the following slides, note the features in common and those which are differentiating. Illustrate each at 400x to take up most of a page. Include and label each listed feature, and give a brief description of its function or significance. Compare with the plates in Eroschenko's Atlas of Normal Human Histology, 9th Ed (VE).
 
 

Thumbnail Image Tissue type, slide, description Features to label in your illustration
1. (slide 2) Simple squamous epithelium, (VE:11): top view of peritoneum, a serous membrane (an example of mesothelium, derived from mesoderm). It functions where a slick friction-free surface is required, or ready exchange across membranes is essential: lung alveoli, capillary endothelium, kidney glomerulus cell borders
nucleus
nucleolus
tiled appearance (its heterogeneity is due to variations in sample preparation)
2. (slide 3) Simple cuboid epithelium, (VE:257) section of kidney. In the cortex (outer portion) of the section, view the proximal convoluted tubules with brush borders. proximal convoluted tubule
basement membrane
nuclei
lumen
brush border (only on proximal convoluted tubules)
3. (slide 4) Simple columnar epithelium, (VE:13) c.s. of the intestine of a Nectarus (a newt).
Note the classic four functional layers of a GI wall, inside to outside: mucosa, submucosa, muscularis, and serosa.
basement membrane
brush border
nuclei
goblet cells
lamina propria (connective tissue which underlies mucous membrane)
capillaries in lamina propria (lacking in epithelium)
4. (slide 5) Stratified squamous epithelium,(VE:17) dog esophagus. This tissue is especially resistant to friction. Note the mucous alveoli of the esophageal glands.  basement membrane
basal cells (brownish cells along basement membrane where mitosis occurs)
squamous cells being shed (superficially, include nucleated surface cells)
lamina propria
5. (slide 6) Pseudostratified ciliated columnarepithelium, c.s. of trachea , (try slide 11 if the detail is poor) (VE:15) This tissue is especially prominent in the respiratory tree. basement membrane
numerous nuclei in deeper half of the tissue
goblet cells
cilia (contrast with brush border for appearance & function)
lamina propria

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