David B. Fankhauser, Ph.D.
Professor of Biology and Chemistry
University of Cincinnati Clermont College
Batavia OH 45103
This page has been accessed times since 27 March 2001.
See Young, Botany Coloring Book,
plates 54, 76, 79.
(Hand out Wet Mount Prep, if not previously given.)
Freshly collected Ebony Spleenwort
dH20 in dropper bottle
scalpel or razor blade
|PREPARED SLIDES (from Carolina Biological):
B 361b Moss antheridium, l.s.
B 363 Moss archegonium, l.s.
B 410 Fern prothallia, antheridia & archegonia
B 415 Fern prothallium with young sporophyte
Make a wet mount of sori: press freshly collected Ebony
fertile leaflet (with sori)
|Take a short field hike to collect fresh Ebony Spleenwort
which carries sori on the underside of its leaflets (note the
rusty lines of sori in the image.
|Take the specimen into the lab inorder to make a wet mount of the sporangia.|
|The equipment for a wet mount include slides, coverslips,
dH2O in a
dropper bottle, a scalpel and a piece of white paper on which to
Here is the protocol
for making a wet mount. Briefly, into
a small drop of H2O, scrape sporangia with the scalpel,
coverslip over it, tap gently to suspend
Draw the following at specified power of magnification:
|View 1: Intact sporangium, annulus, enclosed spores, 100x|
|View 2: Opened sporangium, extended annulus, associated membrane, individual spores, 100x.|
|View 3: Single spore: illustrate a single spore in detail, 400x|
Make illustrations of each of these prepared slides, label structures as listed. If properly planned, all illustrations from a given slide can be drawn on a single page, but make them as large as will fit. Note similarities and differences between the mosses and ferns.
Slide B 361b
View 1. overview showing entire antheridial head, haploid leafy gametophyte, 40x
|View 2. an enlarged antheridium with sperm cells, 100x|
Slide B 363
|View 2. an enlarged archegonium, label stalk, neck cells, egg, 100x|
|This image shows a single fruiting moss plant including the
of the sporophytic stage:
calyptra (white tip on the end of the capsule)
|Here are two views of fruiting moss. Note the required moist habitat, and that the majority of the material shown is the leafy gametophytic state.|
III. FERN SEXUAL REPRODUCTION
[Still need to photograph these
Come back soon.]
|Fern antheridium and
a prothallium (gametophyte)
Slide B 410:
View 1. an entire prothallium, showing rhizoids and archegonia, 40x
|View 2. antheridium detail with sperm cells, 400x|
|View 3. archegonium with neck cells, 400x|
|Fern prothallium with young
slide B415, 40x
prothallium (gametophyte = haploid), antheridia, sporophyte (diploid), root, foot, frond (leaf), vascular tissue
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Send Email to: FANKHADB@UC.EDU