HISTOLOGY OF EPITHELIAL
AND CONNECTIVE TISSUES 
©David B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103
This page has been accessed  times since 21 April 2004. 

24 April 1989, rvsd 3 June '94, 26 Mar '95, 19 Mar 1996, 28 Mar '98, 28 Apr 99, 27 Mar 00, 3 May 01

The following photographs were taken by David Fankhauser using the Olympus C-3000 digital camera through the ocular of a microscopes.  The views are from slides in a set that belongs to our Anatomy and Physiology course. If handled with care, and replaced carefully in their appropriate slots, these slides can be used for years.

Illustrate the tissue structure in the indicated slides at 400x power, no smaller than ½ page each. Make the cells large enough so that they clearly demonstrate the differences between the various tissues. Include and label the features which are listed for each slide. See your biology text book or any A&P text for more information.

For more indepth illustrations and discussion see my page on Epithelial Tissues , and my page on Connective Tissues .

EPITHELIAL TISSUES:
These tissues cover or line organs.  They can be glandular.  They are all attached to a basement membrane, and many have specialized surfaces.  They have an intrinsically high mitotic rate and are prone to forming malignancies (carcinomas).
 
Slide 2. Simple Squamous (top view of peritoneum, a mesothelial abdominal tissue) 

cell boundaries edge of cell membrane 
nucleus holds genetic material 
nucleolus site of RNA synthesis 
cytoplasm 

Slide 3. Simple Cuboid (cross section, from kidney, tubule lining) 

basement membrane found on all epithelial tissues, bonds them to underlying tissue 
duct lumen space within the duct 
nucleus 
brush border (microvilli) proximal convoluted tubules (incr. surf. area) 

Slide 4. Simple Columnar (lining of Nectaris [a newt] intestine) 
The four layers of the gastrointestinal tract, 40x  Outer to inner: 
    serosa 
    muscularis externa 
    submucosa 
    mucosa 
villi
Detail of villus, 100x
basement membrane 
goblet cells in epithelial layer, they make mucous 
microvilli dramatically increase surface area, speeding up absorption 
chromatin stained genetic material within nucleus 
lamina propria connective tissue which underlies all mucous membrane 
Detail of epithelium with goblet cell, 400x
basement membrane 
goblet cells in epithelial layer, they make mucous 
microvilli dramatically increase surface area, speeding up absorption 
chromatin stained genetic material within nucleus 
Slide 5. Esophagus, 40x (dog ) 

stratified squamous epithelium 
basement membrane 
lamina propria underlying mucous membrane 
submucosa filled with esophageal glands (making mucus) 
skeletal muscle (in the dog.  In human it would be smooth muscle)

Stratified squamous epithelium, 400x (lining of dog esophagus) 
flaking off squamous cells 
strata of flattening cells 
stratum basale cells adhered directly to the basement membrane 
basement membrane 
lamina propria below

CONNECTIVE TISSUES:
Connective tissues have a relatively few cells embedded in  intercellular material (matrix).  Matrix consists of protein (often collagen) fibers imbedded in ground substance.  Because connective tissue often requires repair, it can also give rise to malignancies, called sarcomas.
 
. Slide 11. Hyaline cartilage (from tracheal rings in trachea) 

chondrocytes cartilage cells which maintain the tissue 
matrix material in which the chondrocytes are embedded 
lacunae "pools" in which the chondrocytes are housed 
perichondrium connective tissue surrounding the cartilage 

Slide 14. Bone, ground
Multiple Haversian systems, 40x
Haversian systems consist of cells and matrix surrounding central canal 
osteocytes cells which maintain bone, arranged in lamellae 
lamellae concentric circles of bone built up around the central canal 
central canal carries blood vessel supplying nutrients to bone 
Detail of Haversian systems, 100x
whole system of cells and matrix surrounding central canal osteocytes cells which maintain bone 
lamellae concentric circles of bone built up around the central canal 
central canal carries blood vessel supplying nutrients to bone 
Detail of Central Canal and osteocytes, 400x
central canal carries blood vessel supplying nutrients to bone 
osteocytes cells which maintain bone 
canaliculi protoplasmic extensions fr osteocytes into surrounding bone 
lamellae concentric circles of bone built up around the central canal