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30 Oct 01 |
pH
OPTIMUM FOR LACTASE |
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Suspended a 3000 unit tablet in 100
mL 17 Oct 2002. (30 units/mL) |
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Diluted 1.0 mL of the 30 u/mL up to
50 mL with dH2O. Used 0.8 mL per tube |
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(Next year, dilute 0.25 up to 50 mL) |
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Note multiple problems with buffers… |
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| For 2004: |
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A-450: |
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| desired pH for buffers: |
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Group: |
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error |
Measured |
Reported |
I |
II |
III |
IV |
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I |
ratio of A450 |
| difference |
pH |
in pH |
pH |
Tube |
pH |
MG+KG |
RG+SK |
DK+KK |
LP+MS |
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1:5 dil |
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1.5 |
-0.45 |
1.95 |
1 |
2.40 |
0.7 |
0.825 |
0.262 |
0.99 |
0.69425 |
0.137 |
5.109489 |
0.685 |
| 0.8 |
2.3 |
-1.38 |
1.8 |
2 |
3.18 |
0.799 |
0.92 |
0.264 |
0.643 |
0.6565 |
0.139 |
5.748201 |
0.695 |
| 0.7 |
3 |
-1.05 |
3.13 |
3 |
4.18 |
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2.84 |
1.6 |
1.705 |
2.048333 |
0.599 |
0 |
2.995 |
| 0.7 |
3.7 |
-0.80 |
4.2 |
4 |
5.00 |
1.6 |
2.92 |
1.55 |
1.705 |
1.94375 |
0.619 |
2.584814 |
3.095 |
| 0.7 |
4.4 |
-0.70 |
5.4 |
5 |
6.10 |
1.7 |
2.77 |
1.53 |
1.7 |
1.925 |
0.562 |
3.024911 |
2.81 |
| 0.6 |
5 |
-0.13 |
6.72 |
6 |
6.85 |
0.975 |
1.58 |
0.83 |
1.16 |
1.13625 |
0.208 |
4.6875 |
1.04 |
| 0.7 |
5.7 |
-0.32 |
6.88 |
7 |
7.20 |
0.825 |
1.16 |
0.71 |
0.99 |
0.92125 |
0.164 |
5.030488 |
0.82 |
| 0.7 |
6.4 |
0.00 |
7.4 |
8 |
7.40 |
0.561 |
0.679 |
0.455 |
0.649 |
0.586 |
0.099 |
5.666667 |
0.495 |
| 0.7 |
7.1 |
0.02 |
7.65 |
9 |
7.63 |
0.462 |
0.558 |
0.379 |
0.54 |
0.48475 |
0.081 |
5.703704 |
0.405 |
| 0.8 |
7.9 |
0.19 |
8.29 |
10 |
8.10 |
0.285 |
0.322 |
0.243 |
0.315 |
0.29125 |
0.043 |
6.627907 |
0.215 |
| 0.9 |
8.8 |
0.31 |
8.81 |
11 |
8.50 |
0.219 |
0.059 |
0.081 |
0.078 |
0.10925 |
0.004 |
54.75 |
0.02 |
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Temperature of Hot block: |
37.1 |
36.7 |
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31-Oct |
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Buffers |
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1 |
2.4 |
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2 |
3.18 |
0.78 |
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30 |
48 |
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3 |
4.18 |
1 |
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0.625 |
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4 |
5 |
0.82 |
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50 |
1.041667 |
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5 |
6.1 |
1.1 |
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6 |
6.8 |
0.7 |
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7 |
7.2 |
0.4 |
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8 |
7.4 |
0.2 |
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9 |
7.63 |
0.23 |
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10 |
8.1 |
0.47 |
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11 |
8.5 |
0.4 |
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3.5 |
-5 |
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6.85 |
3.35 |
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7.99 |
1.14 |
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current pH |
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altered for |
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better distribution |
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1 |
2.1 |
delta |
2.1 |
delta |
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2 |
2.68 |
0.58 |
2.68 |
0.58 |
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3 |
3.24 |
0.56 |
3.24 |
0.56 |
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4 |
4.25 |
1.01 |
4.1 |
0.86 |
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5 |
4.95 |
0.7 |
4.95 |
0.85 |
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6 |
5.32 |
0.37 |
5.5 |
0.55 |
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7 |
6.31 |
0.99 |
6.1 |
0.6 |
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8 |
6.77 |
0.46 |
6.77 |
0.67 |
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9 |
7.34 |
0.57 |
7.34 |
0.57 |
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10 |
7.93 |
0.59 |
7.93 |
0.59 |
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11 |
8.71 |
0.78 |
8.71 |
0.78 |
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| 7.46 |
0.12 |
10 |
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| 7.74 |
0.06 |
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| 7.87 |
0.13 |
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| 7.68 |
0.22 |
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