30 Oct 01 pH OPTIMUM FOR LACTASE
Suspended a 3000 unit tablet in 100 mL 17 Oct 2002. (30 units/mL)
Diluted 1.0 mL of the 30 u/mL up to 50 mL with dH2O. Used 0.8 mL per tube
(Next year, dilute 0.25 up to 50 mL)
Note multiple problems with buffers…
For 2004:   A-450:
desired pH for buffers: Group:
    error Measured Reported I II III IV I ratio of A450
difference pH in pH pH Tube pH MG+KG RG+SK DK+KK LP+MS 1:5 dil
  1.5 -0.45 1.95 1 2.40 0.7 0.825 0.262 0.99 0.69425 0.137 5.109489 0.685
0.8 2.3 -1.38 1.8 2 3.18 0.799 0.92 0.264 0.643 0.6565 0.139 5.748201 0.695
0.7 3 -1.05 3.13 3 4.18 2.84 1.6 1.705 2.048333 0.599 0 2.995
0.7 3.7 -0.80 4.2 4 5.00 1.6 2.92 1.55 1.705 1.94375 0.619 2.584814 3.095
0.7 4.4 -0.70 5.4 5 6.10 1.7 2.77 1.53 1.7 1.925 0.562 3.024911 2.81
0.6 5 -0.13 6.72 6 6.85 0.975 1.58 0.83 1.16 1.13625 0.208 4.6875 1.04
0.7 5.7 -0.32 6.88 7 7.20 0.825 1.16 0.71 0.99 0.92125 0.164 5.030488 0.82
0.7 6.4 0.00 7.4 8 7.40 0.561 0.679 0.455 0.649 0.586 0.099 5.666667 0.495
0.7 7.1 0.02 7.65 9 7.63 0.462 0.558 0.379 0.54 0.48475 0.081 5.703704 0.405
0.8 7.9 0.19 8.29 10 8.10 0.285 0.322 0.243 0.315 0.29125 0.043 6.627907 0.215
0.9 8.8 0.31 8.81 11 8.50 0.219 0.059 0.081 0.078 0.10925 0.004 54.75 0.02
Temperature of Hot block: 37.1 36.7
31-Oct Buffers
1 2.4
2 3.18 0.78 30 48
3 4.18 1 0.625
4 5 0.82 50 1.041667
5 6.1 1.1
6 6.8 0.7
7 7.2 0.4
8 7.4 0.2
9 7.63 0.23
10 8.1 0.47
11 8.5 0.4
3.5 -5
6.85 3.35
7.99 1.14
current pH altered for 
better distribution
1 2.1 delta 2.1 delta
2 2.68 0.58 2.68 0.58
3 3.24 0.56 3.24 0.56
4 4.25 1.01 4.1 0.86
5 4.95 0.7 4.95 0.85
6 5.32 0.37 5.5 0.55
7 6.31 0.99 6.1 0.6
8 6.77 0.46 6.77 0.67
9 7.34 0.57 7.34 0.57
10 7.93 0.59 7.93 0.59
11 8.71 0.78 8.71 0.78
7.46 0.12 10
7.74 0.06
7.87 0.13
7.68 0.22