MICROBIURET ASSAY OF PROTEIN
	10-Oct
	MICROBIURET STANDARDIZATION
			mg/mL
	tube	dH2O	BSA	A 325
	B	2	0	0.000
	1	1.9	0.1	0.036
	2	1.8	0.2	0.074
	3	1.6	0.4	0.143
	4	1.2	0.8	0.278
	5	0	2	0.560
		Conv Factor	2.797203	mg protein/A 325 unit			A 325	mg/tube	mg/mL	% protein
		specimen	dilution/susp	dH2O	aliquot	micro-	A 325	mg/tube	mg/mL	% protein
			factor			biuret
	blank	blank		2.0		1.0
	1	saliva one	5	1.9	0.1	1.0		0	0	0
	2	saliva one	5	1.0	1.0	1.0		0	0	0
	3	saliva two	5	1.9	0.1	1.0
	4	saliva two	5	1.0	1.0	1.0
	5	solid food	100	1.9	0.1	1.0		0	0	0
	6	solid food	100	1.0	1.0	1.0		0	0	0
	7	liquid food 1	50	1.9	0.1	1.0		0	0	0
	8	liquid food 1	50	1.0	1.0	1.0		0	0	0
	9	liquid food 2	50	1.9	0.1	1.0		0	0	0
	10	liquid food 2	50	1.0	1.0	1.0		0	0	0
	11	BSA mg/mL	50	1.5	0.5	1.0		0	0	0
	12	BSA mg/mL	50	1.0	1.0	1.0		0	0	0
	solid:	Purina Kitten Chow:  (0.39g/39 mL)			liquid:	milk
		IAMS Puppy Food: (0.68 g/68 mL)				egg white
						egg yolk
		specimen	dilution/susp	dH2O	aliquot	micro-	A 325	mg/tube	mg/mL	% protein
			factor			biuret
	blank	blank		2.0		1.0	0.000
	1	saliva WK	5	1.9	0.1	1.0	0.025	0.07	3.5	0.35
	2	saliva WK	5	1.0	1.0	1.0	0.213	0.5964	2.982	0.2982
	3	saliva TM	5	1.9	0.1	1.0	0.159
	4	saliva TM	5	1.0	1.0	1.0	0.641
	5	cat food	100	1.9	0.1	1.0	0.190	0.532	532	53.2
	6	cat food	100	1.0	1.0	1.0	1.140	3.192	319.2	31.92
	7	milk	50	1.9	0.1	1.0	0.101	0.2828	141.4	14.14
	8	milk	50	1.0	1.0	1.0	0.635	1.778	88.9	8.89
	9	egg white	50	1.9	0.1	1.0	0.087	0.2436	121.8	12.18
	10	egg white	50	1.0	1.0	1.0	0.623	1.7444	87.22	8.722
	11	BSA mg/mL	50	1.5	0.5	1.0	0.255	0.714	71.4	7.14
	12	BSA mg/mL	50	1.0	1.0	1.0	0.395	1.106	55.3	5.53
	solid:	Purina Kitten Chow:  (0.39g/39 mL)			liquid:	milk
		IAMS Puppy Food: (0.68 g/68 mL)				egg white
						egg yolk