If we successfully master the prerequisite skills, we will perform the following experiments.
Note that using the new PCR machine may impact on scheduling of listed activities.
 

Tues	Lab Activity	Handouts, Protocols	Illustrations, Data
1/9  Quiz	Drosophila crosses:  Start genetic crosses with Drosophila melanogaster to demonstrate sex linkage. Begin seeds sprouts for next chromosomes Intro to PCR machine.	Genetics Lab Activities Lab Notebook Proc, Format, Cntct Ppr, NB Illus NBGS fr 2/4/97 & 3/11/97 Sprouting Seeds Culturing D. melanogaster Chronlgy of D.m. devel Dissctn Scope Care & Use Sex Linkage in D.m.	vs Drosophila etherizing apparatus Dissecting scope Data: First fly counts D.m. medium prep, your prep, D.m. med Fruit Fly LOG
1/16	Plant chromosomes: in root tips from sprouted seeds  Droso: record status of crosses	BinocMicrosCare,Use&Eval Use of Oil Immersion Obj. Prep of Feulgen Stain Visualizn Root tip chrm	stained root tips Root tip chromos. I Root tip chromos. II
1/23  Quiz	Animal chromosomes: giant polytene chromosomes in Drosophila larvae.	Drosophila Salivary Gland Chromosomes	Score & count F1 Salivary gland illust'n Polytene chromosmes
1/30	Dihybrid cross analysis: corn  Use of 2 test to evaluate data Practice loading gel wells NOTEBOOKS DUE	X2 Probability Table &Samp Gen.Anal. of Hybrid Corn	Corn genetics counts X2 analysis: corn gen
2/6	Electrophoretic sep. of DNA frag's	Electrophoresis, DNAFrags EPh apparat&gel comb ethidium bromide	microcentrifuge & tube Gel reag & plate prep  Gel diagram after run
2/13	DNA digestion: endonucleases Notebooks returned and discussed	NBGS: 2/1/00 notebook Endonuclease Digestion of DNA	rxn tube DNAcalcns DNA dign tube setup
2/20 Quiz	Polymerase Chain Reaction I	Prep of DNA from buccal cells
2/27	Polymerase Chain Reaction II galactosidase assay reagents	Polymerase Chain Reaction Protocol
3/5	Measuring galactosidase in bacterial cultures	Galactosidase in Cells	ONPG hydrolysis gal'ase assay setup tbl
3/7	Gene regulation: induction of the lactose operon NOTEBOOKS DUE	Gene regulation: Induction of lac Operon in E coli	lactose operon galase dereprsn grph Collect lac-grown Ecoli galase SA in E coli

A word about the Lab: Lab time will be used for three purposes:

1) as a recitation session to discuss homework problems and lecture material

2) for the administration of quizzes, 1:00 to 1:30 on noted dates

3) to conduct laboratory work illustrating the principles and lab practices of genetics