Laboratory Schedule for the first half of Microbiology

LAB SCHEDULE FOR MICROBIOLOGY 281
FIRST NOTEBOOK GRADING, SUMMER QUARTER 2005
©David B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103

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Mount this schedule inside the front cover of your Lab Notebook:


DATE	LAB ACTIVITIES	HANDOUTS & PROTOCOLS	ILLUSTRATIONS&DATA
W 7/6	Keeping a notebook Intro to Lab Microscope care & use	Lab Schedule, Part I Syllabus Table of Contents Laboratory Notebook Procedure Table of Contents Format Sugg’t’ns Use of Contact Paper Sample First Notebook Gradesheet Root Beer Protocol Index to Micro Slides Binocular Microscope Use & Care Using and Evaluating Microscope Bacterial Morphology	Diagram of typical NB page Microscope Illustration Bacterial morphologies (3 types at 400x)
F 7/8	Bacterial smear and staining Oil immersion technique	Use of Oil Immersion Objective Bacteriological Smear & Staining Buccal Smear Bacterial Flora of Teeth	Steps of using oil immersion Bacterial morphologies (3 types, 1000x) Bacillus anthracis Buccal Smear Tooth scrapings
M 7/11	LAB FIRST TODAY Prepare a variety of solid media	Commonly Used Micro Media Microbiological Media Prep Autoclave Use	Balance weights, beaker tare Your weights for media prep. Autoclave: numbers on dials
W 7/13	Gram stain Milk Fermenters	Gram Stain Protocol Milk Fermenters	Bacteriological loop Gram stain: yogurt & E. coli Bacteria in fermented milk Gram stain of fecal smear
F 7/15	LAB FIRST Bacterial growth curve If time: Wet mount Sporulating bacteria (prepared slides)	Bacterial Growth Curve Spectrophotometer Use Graph Construction 3 cycle semi-log graph paper Prep. of Wet Mount	Bubbler apparatus Spectrophotometer Growth Curve Readings Bact. growth, linear graph Bact. growth, semi-log graph Bacterial motility Sporulating bacteria
M 7/18	Pipetting practice Serial dilutions	Dilutions Principles Sample Problems Pipetting Practice Serial Dilutions	Pipette diagram, meniscus Diagram of serial dil'n tech. Repipet, settings illustrated Graph, A₆₀₉ vs dye conc.
W 7/20	Sterile technique Plate Spreading Tech. Plate Count (Bring in antiseptic agents for F Lab)	Yeast Plate Count Protocol Sterile Technique: Delivery of Liquids by Pipet Spreading Technique	Repipet w/ means to set vol. Spreader, turntable A₆₆₀ of 10² yeast dilution
F 7/22	Count plates, cal'ns NOTEBOOKS DUE	Bacterial Features, Prepared Slides	yeast plate count data Yeast cells Contaminants of yeast plates Bacterial Features