LAB SCHEDULE FOR MICROBIOLOGY 281, Part II SUMMER 2005Professor of Biology and Chemistry University of Cincinnati Clermont College, Batavia OH 45103 |
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Here
are the laboratory activities for the second half of the quarter of Microbiology
281 at Clermont College.
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DATE
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LAB
ACTIVITIES:
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HANDOUTS
& PROTOCOLS:
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ILLUSTRATIONS
& DATA:
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M
7/25
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Notebooks
returned
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Notebook
gradesheet, 7/23/04
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Make
notes on NB improvement
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Capsules,
etc
(Bring
in antiseptic agents for W Lab)
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Bacterial
Features on Prepared Slides
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capsules
Mycobacteria
tuberculosis
Bacillus subtilis |
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W
7/27
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Growth
inhibition
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Agar
Overlay Technique
Inhibition
of Bact Growth Assay
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Growth
inhibition plate layout
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UV
Killing
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UV
Killing of Bacteria
Additional
Experiments with UV
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UV
plate layout
UV
killing: apparatus
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F
7/29
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Record
inhibition data Discuss significance
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UV
killing results: exposed plate Zones of inhibition, class data
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MIDTERM,
both closed book and open lab book (no loose papers)
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M
8/1
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Blood
agar use
Take
home sterile tube
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beta
Hemolytic Strep Detection
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swabbing
the oropharynx
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W
8/3
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Record
hemolysis plts
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Assay
for Fecal Contamination
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Blood
agar results, illustration
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Coliform
assay: Fecal contam., ambient H2O
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Coliform
data table
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F
8/5
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LAB
1ST: TSI prep
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Triple
Sugar Iron Agar and its Use
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TSI
Slant preparation
Slant solidification configuration |
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Count
colonies
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Class
results, coliform counts
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Streak
out colonies
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Single
Colony Isolation
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Streaking
technique, x4 on one plate
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M
8/8
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TSI
inoculation
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Single
Colony Isolation results
TSI
slant inoculation technique
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W
8/10
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Read
TSI slants
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Triple
Sug. Iron Agar Interpret’n
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TSI
reactions
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Pour
Plates:
Meat
& Milk Contam.
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Pour
Plate Technique
Bact.
Contam. of Milk & Meat
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Dilution
& plating technique:
meat & milk plate counts |
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F
8/12
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Count:Meat&Milk
pltes
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Class
data on meat & milk,
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Protozoan
slides
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Protozoan
and Helminth Parasites
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Protozoan
illustrations
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Take
home sterile bottle
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NOTEBOOKS
DUE
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M
8/15
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Notebooks
returned
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Notebook
gradesheet, 8/12/05
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Memb.
filter assay: Bring in drinking water
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Coliform
in Drinking water, p. 1 Coliform in Drinking water, p. 2
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Membrane
filtration apparatus
Membrane filter plates |
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W
8/17
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LAB
FIRST
virus growth& titering |
Prep.
of Bacterial Virus Stocks Titering of Phage Cultures
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A660
during phage growth
Plating viral dilutions for plaques |
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Count
memb. filters
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Class
Data: drinking water
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F
8/19
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Calculation:
viral titer Phage assay data
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FINAL
EXAM: both closed book and open lab book (no loose papers)
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