Image one	GROWTH REQUIREMENTS, CLASSES OF MEDIA ©David B. Fankhauser, Ph.D., Professor of Biology and Chemistry University of Cincinnati Clermont College, Batavia OH 45103	Image Two
legend for image one	This page has been accessed  times since 17 July 2007. 7/14/83, 7/1/88, 2/28/96, 7/17/96, 7/16/97, 12 July 00, 16 July 01, 16 July 03, 19 July 04, 18 July05, 24 July 06, 16July07	legend for image two

MICROBIAL GROWTH REQUIREMENTS AND MEDIA

Alcamo (5th), pp.106-112, TFC 7 ^th: 156-179, 8^th: 155-179, Black 6^th: 143-169, Bauman 2^nd, pp

NUTRITIONAL REQUIREMENTS:               Water, sine qua non.

 p 167                                                                       CHNOPS, TRACE ELEMENTS (esp Na, K, Ca, Mg, Mn,)

FACTORS AFFECTING GROWTH:

OXYGEN: (169)                       show growth in suspended agar butts:

                                                     Obligate aerobe                            Pseudomonas

                                                     Obligate anaerobe                        Clostridium

                                                     facultative anaerobe                     Escherichia

                                                     microaerophile                             Rhizobium, Neisseria

                                                     Aerotolerant                                 Streptococcus pyogenes

TEMPERATURE: (171)           draw graph, temp vs growth

                                                     psychrophiles    Pseudomonas

                                                     mesophiles         Escherichia, etc

                                                     thermophiles     Thermophiles aquatica (PCR’s Taq enzyme)

pH:                    pH optimum for enzymes determines, vaginal pH due to Lactobacillus , yogurt, pickles, cheese

MOISTURE                               water..., osmosis, halophiles,

OSMOTIC                                 halophiles, preservation in hypertonic solutions (p 155)

CULTURING (p 175)          Colony morphology (p 175)

                                                Inoculation (insert an eye)

                                                Growth of pure cultures (177)

CULTIVATION                   Nutrient broth, agar, enriched media, blood agar, chocolate agar, peptone, tryptone

CHEMICALLY DEFINED (SYNTHETIC), per L                     1 g NH₄H₂PO₄         N and PO₄ source, pH buffer

              p 179:                                                                                   5 g NaCl                   osmolarity

                                                                                                         1 g K₂HPO₄                K and pH buffer

                                                                                                         0.2 g MgSO₄,           Mg and S

COMPLEX (NATURAL) MEDIA:                                             5 g glucose               C source, energy

DIFFERENTIAL media:   (P 181)

              EMB Lac                   eosin and methylene blue inhibit Gm+, 1% lactose. Lac + = purple colonies

              MacConkey agar       lactose and neutral red and crystal violet, bile salts, kills Gm +, Lac+ are red

SELECTIVE MEDIA 

              5% NaCl for Staphylococcus aureus

              EMB lac for Gm- bacterial

ENRICHMENT MEDIA    selenite broth for Salmonella

GROWTH PHASES             lag, log, stationary, decline (= death phase) (p 186)

GENERATION TIME        replication of DNA, time to double number of cells, bacterial fission

                                                     semi-log graphs vs linear graphs (p 186)

ENUMERATION:                plate count, serial dilution                p 188

                                                Filtration                                           p 189

                                                Petroff-Hauser counting chamber    p 190

                                                Most Probable Number                    p 191

                                                Turbidity A₆₆₀                                  p 192

Direct Counts

                                                Activity

                                                Dry weight