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VIRAL DISEASES

7/27/87, rvsd 15 August 1993, 12 Aug 1996, 8/7/98, 7 Aug 06

TFC, P.346, Black's 2nd, p. 266- , Alcamo 323-, Campbell 6^th: , Black’s 6^th: 264-294, tbl: 270

DISCOVERY:

Chamberland                1884     developed porcelain filter to remove bacteria

Iwanowski                    1892     used filter to try to remove tobacco mosaic disease, "filterable virus"

Beijerinck                     1898    showed could be diluted out, destroyed by heat

Forsh & Loeffler          1898    foot and mouth disease caused by filterable agent

Walter Reed                 1901    yellow fever also filterable disease (in Cuba)

Twort & d'Herrelle       1917     bacteriophage

TRAITS: All viruses posses: (p 266)

             capsid             protein coat composed of capsomeres, can contain penetration enzymes

             genome           may be DNA or RNA, double stranded , single stranded, (+ = mRNA) or (-)

Spikes:             glycoprotein for attachment, enzymes to assist attachment

             Host range = which species infected

             specificity = which tissue affected, determined by ability to attach, multiply and release

three morphologies:               icosahedral (20 faces)      icosahedron       herpes, polio, cytomegaly

(p 267 for sizes and shapes)    helical                                      rabies, TMV

                                                 complex                                   small pox, coronavirus, influenza

VARIETY OF VIRUSES, p 270, characterized by comp of genome, enveloped or not, geometry, size

VIRAL REPLICATION:

Obligate Intracellular parasites, replicate inside      absorption

                                                                                 Penetration

                                                                                 Synthesis

                                                                                 Maturation

                                                                                 Release

Bacteriophage parts (p 279): capsid, genome, tail assembly, tail piece, tail fibers, tail sheath, tailcore.  Infection.

             bacteriophage replication: p 280

             lysogeny: p 283 (lysogen. In mammalian cells, called provirus)

enveloped viruses: upon release by budding from host.

             replication of enveloped virus , p 284

             Enveloped viruses     are inactivated by hi temp, hi or lo pH, lipid solv, some disinfectants (Cl, H₂O₂, phenol)

             Naked vs enveloped viruses            naked lack an envelope, resist many of the above

EXAMPLES (perhaps not presented during this lecture?)

COMMON COLD: caused in decreasing frequency by : rhinoviruses, paramyxoviruses, enteroviruses, corona viruses, reoviruses, adenoviruses. Can be mixed infection.

Portal of Entry: mucous membrane of nose and eyes.

Influenza: H = hemagglutanin, N = neuraminidase

                  Alterations in H and N make virus undetected by immune system until after infection

                  1918: Change to H3N2, killed 20 million world wide. More troops died of flu than combat.  Capable of recombination.

Herpes     form provirus, integrated for life into your DNA: Chicken Pox, Herpes simples I and II

HIV          Primarily parenteral POE, through wound or sore. Infects T cells. Forms provirus.

TABLE SUMMARIZING MAJOR CLASSES OF VIRUSES

David B. Fankhauser, PhD

15 August 1993, rvsd 12 August 1994, 15 Feb 02, 15 Feb 06, 26Apr06, 7 Aug 06, 8 Aug07

Bauman 2^nd: 680-740