MICROBIOLOGY 34-BIOL-281-151 SUMMER QUARTER, 2004 SYLLABUS ©David B. Fankhauser, Ph.D., Professor of Biology and Chemistry University of Cincinnati Clermont College,  Batavia OH 45103
Plate spreading technique	Office: EDS 215 P.  Office Hours: MWF, 1:15-3 PM email: David.Fankhauser@UC.EDU This page has been accessed  times since 1 July 2004.	Testing drinking water by membrane filtration

COURSE OBJECTIVES:
To learn about microorganisms, including their anatomy, physiology, taxonomy, genetics, how to control their growth, their medical significance, their epidemiology, the body's protective responses to their challenge (both non-specific and immune), exogenous antimicrobial agents, and etymology related to all these subject.

LABORATORY SKILLS TO BE LEARNED:
oil immersion microscopy , differential staining , sterile technique , media preparation , single colony isolation , serial dilution , use of selective and differential media , microbial enumeration: (photometrically , plate count , membrane filtration ),  inhibition assay .

See Lab Notebook Procedure for important details.  Here is the Schedule of Lab Activities for Microbiology .

REQUIRED TEXT:     Tortora, Funke & Case  Microbiology, An Introduction,            8th Ed    Benjamin/Cummings Pub.    (2004) 

OPTIONAL TEXTS:  Borror                              Dict. Of Word Roots & Comb Forms                 Mayfield Pub.                         (1960)
                                       Marchuk                          A Life Science Lexicon                                         Wm. C. Brown Pub                (1992)

REQUIRED MATERIALS FOR THE LAB (BRING THE FIRST DAY):
              1) Pen, permanent black: Pilot Precise V5, Uniball Micro, or Tombo Fine. 
              2) Notebook, graph-lined bound Composition, 10 x 7 7/8   (All notes taken during Lab should be entered directly into this book.)

DAILY SCHEDULE:      Lecture is 10:00 to approximately 11:25
                                           Laboratory from 11:35 to 1:15.
This calendar is an approximation, and may be adjusted according to the rate at which material is successfully presented and mastered.
Assigned readings: page numbers in lower left boxes. For details on specific diseases, see text index.

READINGS IN TEXT ARE IN LOWER LEFT OF DUE DATE.
NOTE:Pop pre-lab quizzes will be given.

7/5          NATIONAL HOLIDAY	7/7 History of Microbiology Spontaneous Generation? 1-25	7/9 (Review Chemistry on your own) Germ Theory of Disease Microscope History & Function 26-67
7/12Lab First: Prepare media Stains Prokaryotic Anatomy I: Capsules, Flagella, Pili 67-82	7/14 Prokaryotic Anatomy II: Cell Wall Structure, Sporulation 82-110                            QUIZ I	7/16 Lab First, middle and last: Bacterial Growth Curve Enzymology, Metabolism, Glycolysis 111-125
7/19 Microbial Fermentation Requirements for Microbial Growth 125-154 155-182	7/21 Microbial Control: Physical Means   183-193                          QUIZ II	7/23 Microbial Control: Chemical Means (bring in anti-bacterial agents to test) 193-209,559-589 NOTEBOOKS DUE
7/26 Microbial Genetics   210-247	7/28 MicrobialTaxonomy Intro to Gram negative bacteria 276-313	7/30Lab First: Blood Agar results Take home sterile culture tubes MIDTERM EXAM
8/2 Midterm returned, discussed. Gram negative bacteria continued: Enterobacteriaceaeet cetera 313-319	8/4 Chlamydia & Spirochetes Gram positive bacteria: Staph, Strep,et cetera 319-333	8/6Lab First: TSI slant prep Spore formers: Bacillus & Clostridium Mycobacteria: TB and leprosy 446-447, 623, 683                  QUIZ III
8/9 Medically Important Fungi Eukaryotic Parasites 334-345, 352-375	8/11 Viruses, Viral Diseases I: Viral diseases of skin and nerves 376-407, 593, 599-605, 625-635	8/13 Virus Diseases II: Resp. & GI tracts: 691-694, 720-726 Infection and Disease: 408-436 NOTEBOOKS DUE               QUIZ IV
8/16 Host Defenses: Non-specific and specific 458-481	8/18 Immunology: Mech. of the Immune Response, Problems 482-507	8/20Lab 1 st: Count plaques, calc. titer FINAL EXAM So long, it's been good to know you. KEEP IN TOUCH!

Quizzes (given at 11:00) and exams will include material from both lecture and lab exercises. Grades will be assigned according to your position on a class histogram of cumulative points earned on quizzes, notebooks and exams. The class midline approximates a B. See separate handout on suggestions for how to succeed in this course.
Version 30 June 2004