COURSEOBJECTIVES: To learn about microorganisms, including their anatomy, physiology, taxonomy, genetics, how to control their growth, their medical significance, their epidemiology, the body's protective responses to their challenge (both non-specific and immune), exogenous antimicrobial agents, and etymology related to all these subject.
 

LABORATORYSKILLS TO BE LEARNED: oil immersion microscopy, differential staining, sterile technique, media preparation, single colony isolation, serial dilution, use of selective and differential media, microbial enumeration (photometrically, plate count, membrane filtration), inhibition assay. See Lab Notebook Procedure for important details.

REQUIRED TEXT: Tortora, Funke & Case Microbiology, An Introduct'n, 8th Ed Benjamin/Cummings Pub. (2004)

OPTIONAL TEXTS: Borror Dict. Of Word Roots & Comb Forms Mayfield Pub. (1960)
Marchuk A Life Science Lexicon Wm. C. Brown Pub (1992)
 

SCHEDULE: Lecture is 10:00 to approximately 11:25, Laboratory from 11:35 to 1:15.

This calendar is an approximation, and may be adjusted according to the rate at which material is successfully presented and mastered. Assigned readings: page numbers in lower left boxes. For details on specific diseases, see text index.
 

READINGS IN TEXT ARE IN LOWER LEFT OF DUE DATE. 
NOTE: Pop pre-lab quizzes will be given.

7/5	7/7 History of Microbiology Spontaneous Generation? 1-25	7/9  (Review Chemistry on your own) Germ Theory of Disease  Microscope History & Function  26-67
7/12 Lab First: Prepare media  Stains Prokaryotic Anatomy I: Capsules, Flagella, Pili 67-82	7/14 Prokaryotic Anatomy II:  Cell Wall Structure,  Sporulation 82-110 QUIZ I	7/16  Lab First, middle and last: Bacterial Growth Curve Enzymology, Metabolism, Glycolysis 111-125
7/19  Microbial Fermentation  Requirements for Microbial Growth 125-154 155-182	7/21 Microbial Control: Physical Means 183-193 QUIZ II	7/23  Microbial Control: Chemical Means (bring in anti-bacterial agents to test) 193-209,559-589 NOTEBOOKS DUE
7/26  Microbial Genetics     210-247	7/28  Microbial Taxonomy Intro to Gram negative bacteria 276-313	7/30 Lab First: Blood Agar results  Take home sterile culture tubes MIDTERM EXAM
8/2 Midterm returned, discussed. Gram negative bacteria continued: Enterobacteriaceae et cetera 313-319	8/4 Chlamydia & Spirochetes Gram positive bacteria: Staph, Strep, et cetera 319-333	8/6 Lab First: TSI slant prep  Spore formers: Bacillus & Clostridium Mycobacteria: TB and leprosy 446-447, 623, 683 QUIZ III
8/9 Medically Important Fungi Eukaryotic Parasites 334-345, 352-375	8/11 Viruses, Viral Diseases I: Viral diseases of skin and nerves 376-407, 593, 599-605, 625-635	8/13 Virus Diseases II: Resp. & GI tracts: 691-694, 720-726 Infection and Disease: 408-436 NOTEBOOKS DUEQUIZ IV
8/16 Host Defenses:  Non-specific and specific  458-481	8/18 Immunology: Mech. of the Immune Response, Problems  482-507	8/20 Lab 1st: Count plaques, calc. titer  FINAL EXAM So long, it's been good to know you. KEEP IN TOUCH!

Quizzes (given at 11:00) and exams will include material from both lecture and lab exercises. Grades will be assigned according to your position on a class histogram of cumulative points earned on quizzes, notebooks and exams. The class midline approximates a B. See separate handout on suggestions for how to succeed in this course.

Version 30 June 2004