MICROBIOLOGY 34-BIOL-281-151 SUMMER QUARTER, 2005 SYLLABUS ©David B. Fankhauser, Ph.D., Professor of Biology and Chemistry              University of Cincinnati Clermont College,  Batavia OH 45103
Plate spreading technique	Office: EDS 215 P.  Office Hours: MWF, 1:15-3 PM This page has been accessed  times since 17 June 2005.	Testing drinking water by membrane filtration

UNDER CONSTRUCTION: Page numbers in the text book by Black will be entered soon.

COURSE OBJECTIVES:
To learn about microorganisms, including their anatomy, physiology, taxonomy, genetics, how to control their growth, their medical significance, their epidemiology, the body's protective responses to their challenge (both non-specific and immune), exogenous antimicrobial agents, and etymology related to all these subject.

LABORATORY SKILLS TO BE LEARNED:
oil immersion microscopy, differential staining, sterile technique, media preparation, single colony isolation, serial dilution, use of selective and differential media, microbial enumeration: (photometrically, plate count, membrane filtration), inhibition assay. See Lab Notebook Procedure for important details.

REQUIRED TEXT:  Black, Jacquelyn G., Microbiology Principles and Explorations, 6th Ed., Wiley, (2005)

OPTIONAL TEXTS: Borror, Dict. Of Word Roots & Comb Forms Mayfield Pub. (1960)

SCHEDULE:Lecture is 10:00 to approximately 11:25, Laboratory from 11:35 to 1:15.
This calendar is an approximation, and may be adjusted according to the rate at which material is successfully presented and mastered. Assigned readings: page numbers in lower left boxes. For details on specific diseases, see text index.

READINGS IN TEXT ARE IN LOWER LEFT OF DUE DATE.
NOTE:Pop pre-lab quizzes will be given.

MONDAY	WEDNESDAY	FRIDAY
7/4 INDEPENDANCE DAY	7/6 History of Microbiology Spontaneous Generation?	7/8 (Review Chemistry on your own) Germ Theory of Disease Microscope History & Function
7/11  Lab First:  Prepare media     LECTURE:Prokaryotic Anat I: Stains; Capsules, Flagella, Pili	7/13 Prokaryotic Anatomy II: Cell Wall Structure, Sporulation                            QUIZ I	7/15 Lab First, middle and last:                      Bacterial Growth Curve            LECTURE: Enzymology, Metabolism, Glycolysis
7/18 Microbial Fermentation Requirements for Microbial Growth	7/20 Microbial Control: Physical Means                             QUIZ II	7/22 Microbial Control: Chemical Means (bring in anti-bacterial agents to test)  NOTEBOOKS DUE
7/25 Microbial Genetics	7/27 Microbial Taxonomy Intro to Gram negative bacteria	7/29   Lab First: Blood Agar results           Take home sterile culture tubes MIDTERM EXAM
8/1 Midterm returned, discussed. Gram negative bacteria continued: Enterobacteriaceaeet cetera	8/3 Chlamydia & Spirochetes Gram positive bacteria: Staph, Strep,et cetera	8/5  Lab First: TSI slant prep                      Spore formers: Bacillus & Clostridium Mycobacteria: TB and leprosy                  QUIZ III
8/8 Medically Important Fungi Eukaryotic Parasites	8/10 Viruses, Viral Diseases I: Viral diseases of skin and nerves	8/12 Virus Diseases II: Resp. & GI tracts:  Infection and Disease:  NOTEBOOKS DUE               QUIZ IV
8/15 Host Defenses: Non-specific and specific	8/17 Immunology: Mech. of the Immune Response, Problems	8/19  Lab 1st: Count plaques, calc. titer        FINAL EXAM So long, it's been good to know you. KEEP IN TOUCH!

Quizzes (given at 11:00) and exams will include material from both lecture and lab exercises. Grades will be assigned according to your position on a class histogram of cumulative points earned on quizzes, notebooks and exams. The class midline approximates a B. See separate handout on suggestions for how to succeed in this course.
Version 17 June 2005