MICROBIOLOGY

SUMMER QUARTER, 2003
David B. Fankhauser, Ph.D.
Professor of Biology & Chemistry

 
 
Office, Hrs:
     EDS 215 P (MWF, 1:15-3 PM)
Homepage: 
     http://biology.clc.uc.edu/Fankhauser
email:
     David.Fankhauser@UC.EDU
34-BIOL-281
SYLLABUS
U. C. Clermont College                 
Batavia  OH 45l03
 
COURSE OBJECTIVES:
To learn about microorganisms, including their anatomy, physiology, taxonomy, genetics, how to control their growth, their medical significance, their epidemiology, the body's protective responses to their challenge (both non-specific and immune), exogenous antimicrobial agents, and etymology related to all these subject.

LABORATORY SKILLS:

To learn these skills: oil immersion microscopy, differential staining, sterile technique, media preparation, single colony isolation, serial dilution, use of selective and differential media, enumeration (photometrically, plate count, membrane filtration).See Lab Protocol handouts for important directions on Lab Notebook Procedure.

REQUIRED TEXT:     Tortora, Funke & Case Microbiology, An Introduct’n, 7th EdBenjamin/Cummings Pub.(2001)

OPTIONAL TEXTS:    BorrorDict. Of Word Roots & Comb FormsMayfield Pub.(1960)

                                       MarchukA Life Science LexiconWm. C. Brown Pub(1992)
 
SCHEDULE:Lecture is 10:00 to approximately 11:25,Laboratory from 11:35 to 1:15.
The following calendar is an approximation, and may be adjusted according to the rate at which material is successfully presented and mastered.Box lower left numbers are assigned readings.For details on specific diseases, see text index.

READINGS IN TEXT ARE IN LOWER LEFT OF DUE DATE.NOTE: Pop pre-lab quizzes will be given.

6/30       History of Microbiology
Spontaneous Generation?
1-28
7/2(Review Chemistry on your own)
Germ Theory of Disease 
Microscope History & Function 
29-83
7/4
Independence Day
7/7Lab First: Prepare media
Stains.
Prokaryotic Anatomy I:  Flagella, Pili
85-94 
7/9 Prokaryotic Anatomy II:
Capsules, Cell Wall Structure,
Sporulation
94-106                                                 QUIZ I
7/11 Lab First, middle and last:
Bacterial Growth Curve
Requirements for Microbial Growth
106-119
7/14       Enzymology
Metabolism, Glycolysis
121-135
7/16       Microbial Fermentation 
Microbial Control: Physical Means
135-154                                             QUIZ II
7/18 Microbial Control:
Chemical Means
(bring in anti-bacterial agents to test)
647-721NOTEBOOKS DUE
7/21 
Microbial Genetics
155-191
7/23       Microbial Taxonomy
Intro to Gram negative bacteria
304-314
7/25 Lab First:  Blood Agar results 
Take home sterile culture tubes
MIDTERM EXAM
7/28  Midterm returned, discussed.

Gram negative bacteria continued:

Enterobacteriaceae et cetera
7/30   Chlamydia & Spirochetes
Gram positive bacteria:
Staph, Strep, et cetera
582-586, 658-660, 690-691
8/1 Lab First: TSI slant prep
Spore formers: Bacillus & Clostridium
Mycobacteria
324-325,                                            QUIZ III
8/4     Medically Important Fungi
Eukaryotic Parasites
331-343, 350-354
8/6       Viruses, Viral Diseases I:
Viral diseases of skin and nerves
587-593, 611-620
8/9       Virus Diseases II:
Respiratory & GI tracts: 371-402
Infection and Disease: 406-425
NOTEBOOKS DUE                      QUIZ IV
8/11       Host Defenses:
Non-specific and specific 
454-472
8/13       Immunology:
Mech. of the Immune Response, Problems 
476-495
8/15 Lab 1st: Count plaques, calc. titer
FINAL EXAM
So long, it's been good to know you.
KEEP IN TOUCH!

Quizzes (given at 11:00) and exams will include material from both lecture and lab exercises.Grades will be assigned according to your position on a class histogram of cumulative points earned on quizzes, notebooks and exams.The class midline corresponds to a B/B-See a separate handout on suggestions for how to succeed in this course.

Rvsd 25 June 2003