Micro Class, Spring Semester 2013

MICROBIOLOGY 2031

Syllabus and Laboratory Handouts
  Fall Semester
ęDavid B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
             University of Cincinnati Clermont College, 
Batavia OH 45103
Microbiology class, Fall Semester 2012
Microbiology Students,
Spring Semester 2013
File "Micro_handouts.htm" was last modified on 27 Dec 2013.File "Micro_handouts.htm" was last modified on 27 Dec 2013.File "Micro_handouts.htm" was last modified on 27 Dec 2013.File "Micro_handouts.htm" was last modified on 26 Apr 2013.
is page has been accessed Counter times since 18 July 2001.
Microbiology Students,
Fall Semester 2012

Here are the grades for 2013 Spring Microbiology according to UC "M" number.

Here are pdfs for lecture notes and lab protocols (please do not reproduce without written permission):

LECTURE NOTES:  Here is a directory of informal lecture notes for my course.  Please read the paragraph below.**
LABORATORY PROTOCOLS AND HANDOUTS:  a directory of lab handouts.
Grades for 2012 Autumn Microbiology will be linked here at the end of the quarter according to UC "M" number..


BELOW ARE ILLUSTRATED PAGES FOR THE HANDOUTS CREATED FOR THIS COURSE.
PLEASE DO NOT REPRODUCE WITHOUT WRITTEN PERMISSION.
The following protocols have been written by David Fankhauser for his Microbiology Course at the University of Cincinnati Clermont College.
They should not be reproduced without his express written permission. Thank you. Links?
1
2
3
4
5
6
7

9
10
11
12
13
14
15
 15b
16
17a
17b
18a
 18b
19
20
21
22
23
24
25
26
27
28
29
30

31
32& 33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69

Table of Contents
Micro 2031 Spring Semester Course Syllabus                                  (Here is a previous syllabus listing pages in Bauman's 2nd Edition...)
How to Take a Fankhauser Course
Study Groups: Towards Effective Peer Education
Study Group Report Form
Micro Wordstems I, first half of quarter
Micro Wordstems II, second half of quarter
Micro Cumulative Wordstems List with meanings
FOR THE LAB NOTEBOOK:
Lab Schedule for Microbiology 2031, Spring 2013, FIRST HALF
Laboratory Notebook Procedure  (Here is a pdf of the handout, 2012)            Here is an illustrated page devoted to setting up your Microbiology Lab book.
Format for Table of Contents
Use of Contact Paper for Mounting Handouts
Sample First Notebook Gradesheet from previous year
Notebook Illustrations
Making Root Beer at Home
Bacterial Colonies on Potato Slices Ó la Koch
Index to Micro Slides

Binocular Microscope: Its Features and Care
Microscope Storage Gradesheet
How to View a Slide
Bacterial Morphology for Microbiology Students
Sample Math Problems for Microbiology
Use of Oil Immersion Objective
Equipment for a Microbiology Work Station
Bacteriological Smear and Staining Protocol
Buccal Smear
Bacterial Flora of Teeth
Microbiological Media Preparation
Commonly Used Solid Media and Other Common Microbiological Media
Other Commonly Used Media for Phage Growth
Autoclave Use
Bacterial Growth Curve: E coli on various types of liquid media
Spectrophotometer Use
Graph Construction  (Here is a pdf of an example notebook page demonstrating Graph Construction.)
Two sheets of three cycle semi-log paper
Preparation of Wet Mount Slide
Gram Stain Protocol
Milk Fermenters
Dilutions with Sample Problems
Serial Dilution, Pipetting Practice
Displacement Pipetters: Their Care and Use
Sterile Technique: Delivery of Liquids by Pipet 
Spreading Technique for Plating Bacteria
Yeast Plate Count Protocol 
Bacterial Anatomy (on Prepared Slides)

Table of Contents, Part 2
Lab Schedule for Microbiology 2031, Second half
Microbiology Wordstems, Second Half of Quarter
Sample Second Note Book Grade Sheet from previous year
Agar Overlay Technique
Demonstration of Bacterial Growth Inhibition
UV Killing of Bacteria
Additional Experiments with the UV Killing Assay
Hemolytic Streptococcus Detection by Throat Culture
Assay for Coliform Contamination in Ambient Water
Single Colony Isolation
Triple Sugar Iron Agar and Its Use
Triple Sugar Iron Agar: Interpretation of Results
Pour Plate Technique for Bacterial Enumeration
Bacterial Contamination of Milk: Pour Plate Assay
Bacterial Contamination of Meat: Pour Plate Assay
Meat and Milk Assay Setup
Protozoan and Helminth Parasites
Protozoan Phyla and Examples (not required for NB)
Salmonella Detection
Preparation of Media for Salmonella Detection
Coliform in Drinking Water Assay, p 1 
Coliform in Drinking Water Assay, p 2
Preparation of Bacteriophage Stocks
Titering of Bacterial Viruses

LINKS:  Questions about microbes?  Here is a rich resource page of information about microbes at microbes.info

**Here is a directory containing some of my lecture notes in *.pdf form.  Note that they are outline form, and informal.  If you see errors in them please let me know.Even with these notes printed out, I urge students to take their own detailed notes in class for several reasons.  First, the act of writing is very important in creating a long term memory in your brain.  Second, my lecture notes are only guidelines for my lectures.  My students soon recognize that I often go on a tangent which may not be in my prepared notes.  Third, when you review your own handwritten notes, you will see areas which are not clear, and provide you an opportunity to clarify the question at the beginning of the next class.

And here is the directory of *pdf files of my Microbiology Lab protocols.


 The following are pages which contain links to web images related to lectures presented. 

The History of Microbiology.
The History of the Germ Theory of Disease.
Essentials of microscopy and staining.
Prokaryotic Anatomy
Enzymology and Metabolism
Catabolism
Microbial Fermentation (Part of previous page...)
Requirements for Microbial Growth and Media
Control of Microbial Growth, Physical Means
Control of Microbial Growth, Chemical Means
Microbial Genetics
Microbial Taxonomy
Staphylococci and Streptococci
Spore Forming Bacteria
Gram Negative Rods and Cocci
Mycoplasmas, Rickettsias, Chlamydias, Spirochetes, Vibrios
Mammalian Pathogenic Viruses
Disease Processes
Non-specific Defences
Immunology

Here is a page of illustrations related to the history of microbiology.

Here are collages of students from previous years:

Microbiology, Summer 2003
Microbiology,
                    Summer 2004 2005 Summer
                Microbiology class 2005 Summer
                Microbiology class
Microbiology, Summer
                  2007

Microbiology Students,
Summer 2002
Microbiology Students,
Summer 2003
Microbiology Students,
Summer 2004
Microbiology Students,
Summer 2005
Microbiology Students,
Summer 2006

Microbiology, Summer
                  2007
Micro class, Summer 2008
2009 Microbiology
Micro Class, Summer 2010
Summer Microbiology Class, 2011

Summer 2007
Summer 2008 (large image)
Summer 2009 (large image)
Summer 2010 (large image) Microbiology Students,
Summer 2011


  Summer 2001 class