Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103
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11 August 1995, 23 July 96, 16 August 96, 22 July 97, 19 July 98, 7 Aug 00, 16 July01
See Alcamo, 4th , p 451-465, Campbell's 5th, pp 524-528, Tortora, Funke & Case,7th, pp 349-366.
Illustrate the protozoans (first three
specimens) at 1000x, and the last, a nematode
Trichinella, at 400x.
As always, scan at lower power to find a field rich in organisms. Take
care to maintain correct scale. Label features which can be observed, note
diseases they cause.
MASTIGOPHORA: Class of whip-bearing
protozoans, in kingdom protista, phylum protozoa
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Slide 22 Trypanosoma gambiense
(PS 310)
(or view Trypanosoma lewesi PS 300, harder to see) In an infected blood smear, among the erythrocytes (RBCs), wavy organisms with nucleus, flagella and (hard to see) undulating membrane. A member of the kinetoplastids. sleeping sickness, spread by tsetse fly in Central Africa. Chancre at bite, fever, facial edema, lymphadenopathy, CNS symptoms, fatal coma. |
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Slide 19 Giardia lamblia
(PS 210)
In an infected fecal smear. Bilateral trophozoites: two cells adhered looks like a tear drop with two nuclei appearing like eyes. Note "old man" appearance due to diploid structure. The eyes are the nuclei of two cells. giardiasis: Most common protozoal disease, 4% of USA population are infected, commonly asymptomatic. Causes small intestine enteritis (nausea, flatulence, eructation, foul smelling diarrhea). Prevents absorption of nutrients, passes in feces as cysts, human and wild animals. One cause of traveler's diarrhea, not killed by Cl2. |
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Slide 20 Trichomonas vaginalis
(PS 250)
In an infected vaginal smear: Roughly circular, nucleus is barely visible within cell. The flagellum is only occasionally visible. Note squamous cells and polymorphonucleocyts. vaginitis, found in GU of & . Causes pruritus, copious green/yellow frothy discharge (ingests lactobacilli which raises vaginal pH from 3 to 4.5). 30% of pop colonized. STD, spread also on towels, toilet facilities, very sensitive to drying |
PHYLUM NEMATODA (Kingdom Animalia)
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Slide 24 Trichinella spiralis
(PS 2430)
At 100x or 400x: Illustrate several cysts in striated muscle. Note numerous sections through the coiled nematode. The roundworm is suspended in a jelly-like material within the capsule. trichinosis: caused by a eukaryote belonging to the phylum Nematoda. Encysted larvae in pork or bear meat, and flesh of other carnivores. Mate in intestine, lays living larvae. Cross GI track, migrate. Can cause heart, kidney failure. (>90% of infected persons carry Ab.) Only 150 cases/yr in USA. |
